Poly(A)-specific ribonuclease (PARN) mediates 3′-end maturation of the telomerase RNA component
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چکیده
منابع مشابه
A Polyadenylation-Dependent 3' End Maturation Pathway Is Required for the Synthesis of the Human Telomerase RNA.
Telomere maintenance by the telomerase reverse transcriptase requires a noncoding RNA subunit that acts as a template for the synthesis of telomeric repeats. In humans, the telomerase RNA (hTR) is a non-polyadenylated transcript produced from an independent transcriptional unit. As yet, the mechanism and factors responsible for hTR 3' end processing have remained largely unknown. Here, we show ...
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Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC) complementary to the human ...
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Transcripts encoded by 2 different rat genomic clones, rg13 and rg100, appear to be typical brain-specific polyA- RNAs, as defined by previous criteria (rare, polysomal, and postnatally expressed from single-copy genes). However, we have found by using a sensitive nuclease protection assay that low levels of these transcripts (10% and 3%, respectively) are detected in polyA+ RNA. To determine i...
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Elongation of human telomeres requires the enzymatic activity of the telomerase holoenzyme. In vitro, telomerase activity can be reconstituted by the catalytic subunit (hTERT) and the RNA subunit (called hTERC, hTER, or hTR). However, the routes of the RNA and protein components of telomerase from their generation to assembly into telomerase appear to be complex and likely to be highly regulate...
متن کاملInteraction of the 3'-end of tRNA with ribonuclease P RNA.
Ribonuclease P, which contains a catalytic RNA subunit, cleaves 5' precursor-specific sequences from pre-tRNAs. It was previously shown that the RNase P RNA optimally cleaves substrates which contain the mature, 3'-terminal CCA of tRNA. In order to determine the contributions of those individual 3'-terminal nucleotides to the interaction, pre-tRNAs that have CCA, only CC or C or are without CCA...
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ژورنال
عنوان ژورنال: Nature Genetics
سال: 2015
ISSN: 1061-4036,1546-1718
DOI: 10.1038/ng.3423